Amanda Ellen Ramer-Tait |
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CURRICULUM VITAE |
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University of Nebraska-Lincoln |
Phone: (402) 472-7293 |
APPOINTMENTS |
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| 2012-Present |
Assistant Professor Department of Food Science and Technology, University of Nebraska-Lincoln Courtesy Assistant Professor School of Biological Sciences, University of Nebraska-Lincoln Courtesy Assistant Professor School of Veterinary Medicine and Biomedical Sciences, Univ. of Nebraska-Lincoln Collaborator Assistant Professor Department of Veterinary Microbiology and Preventive Medicine, Iowa State Univ. |
| 2011-2012 |
Associate Scientist Department of Veterinary Microbiology and Preventive Medicine, Iowa State Univ. |
| 2008-2012 |
Assistant Scientist II |
EDUCATION |
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| 2006-2008 |
Postdoctoral Associate, Laboratory of Dr. Jeffrey Beetham Department of Veterinary Pathology, Iowa State University, Ames, IA |
| 2000-2006 |
Ph.D. in Immunobiology, Laboratory of Dr. Douglas Jones Department of Veterinary Pathology, Iowa State University, Ames, IA |
| 1996-2000 |
B.S. in Biochemistry, Minor in Agriculture (Animal Science emphasis) Western Kentucky University, Bowling Green, KY |
AWARDS AND HONORS |
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| 2012 | Crohn's and Colitis Foundation of America Career Development Award |
| 2006 |
Iowa State University Research Excellence Award for outstanding research contributions by a graduate student |
| 2001 | Gamma Sigma Delta |
| 1998 | Phi Kappa Phi Honor Society |
PUBLICATIONS-Peer-Reviewed Journal Articles |
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1. Carrillo-Conde BR, Darling RJ, Seiler SJ, Ramer-Tait AE, Wannemuehler MJ, Narasimhan B. Sustained release and stabilization of therapeutic antibodies using amphiphilic polyanhydride nanoparticles. Accepted. Chemical Engineering Science. |
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2. Huntimer LM, Ross KA, Darling RJ, Winterwood NE, Boggiato P, Narasimhan B, Ramer-Tait AE, Wannemuehler MJ. Polyanhydride nanovaccine platform enhances antigen-specific cytotoxic T cell responses. Accepted. Technology. |
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3. Phanse Y, Carrillo-Conde BR, Ramer-Tait AE, Broderick S, Kong CS, Rajan K, Flick R, Mandell RB, Narasimhan B, Wannemuehler MJ. A systems approach to designing next generation vaccines: combining α-galactose modified antigens with amphiphilic nanoparticle platforms. 2014. Nature Sci Rep. 4:3775. |
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4. Knetter SM, Bearson SMD, Huang TH, Kurkiewicz D, Schroyen M, Nettleton D, Berman D, Cohen V, Lunney JK, Ramer-Tait AE, Wannemuehler MJ, Tuggle CK. Salmonella enterica serovar Typhimurium-infected pigs with 1 different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes. 2014. Innate Immunity. Mar 14. Epub ahead of print. |
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5. Knetter SM, Tuggle CK, Wannemuehler MJ, Ramer-Tait AE. Swine barn dust extract exposure impairs porcine macrophage function in vitro: Implications for respiratory health. 2014. Vet Immunol Immunopathol. 157(1-2):20-30. |
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6. Phanse Y, Carrillo-Conde BR, Ramer-Tait AE, Roychoudhuri R, Pohl NLB, Narasimhan B, Wannemuehler MJ, Bellaire BH. Functionalization of polyanhydride microparticles with di-mannose influences uptake by and intracellular fate within dendritic cells. 2013. Acta Biomaterialia. 9(11):8902-8909. |
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7. Haughney SL, Petersen LK, Schoofs A, Ramer-Tait AE, King J, Briles D, Wannemuehler, Narasimhan B. Retention of structure, antigenicity, and biological function of pneumococcal surface protein A (PspA) released from polyanhydride nanoparticles. 2013. Acta Biomaterialia. 9(9):8262- 71. |
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8. Petersen LK, Huntimer L, Walz K, Ramer-Tait A, Wannemuehler MJ, Narasimhan B. Combinatorial evaluation of in vivo distribution of polyanhydride particle-based platforms for vaccine delivery. 2013. Int J Nanomedicine. 8:2213-2225. |
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9. Huntimer L, Wilson Welder JH, Ross K, Carrillo-Conde B, Pruisner L, Narasimhan B, Wannemuehler MJ, Ramer-Tait AE. Single immunization with a suboptimal antigen dose encapsulated into polyanhydride microparticles promotes high titer and avid antibody responses. 2013. J Biomed Mater Res Part B. 101(1):91-98. |
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10. Huntimer L, Ramer-Tait AE, Petersen L, Ross K, Walz K, Wang C, Hostetter J, Narasimhan B, Wannemuehler MJ. Evaluation of biocompatibility and administration site reactogenicity of polyanhydride particle-based platform for vaccine delivery. 2013. Adv Healthc Mater. 2(2):369-78. |
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11. Ramer-Tait AE, Lei SM, Bellaire BH, Beetham JK. Differential surface deposition of complement proteins on logarithmic and stationary phase Leishmania chagasi promastigotes. 2012. J Parasitol. 98(6):1109-16. |
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12. Carrillo-Conde B, Ramer-Tait AE, Wannemuehler MJ, Narasimhan B. Chemistry-dependent adsorption of serum proteins onto polyanhydride microparticles differentially influences uptake and activation mechanisms of dendritic cells. 2012. Acta Biomater. 8(10):3618-28. |
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13. Wilke V, Nettleton D, Wymore M, Gallup JM, Demirkale CY, Ackermann M, Tuggle C, Ramer-Tait AE, Wannemuehler MJ, Jergens AE. 2012. Mucosal gene expression in dogs with chronic enteropathies. Am J Vet Res. 73(8):1219-29. |
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14. Phanse Y, Ramer-Tait AE, Friend SL, Carrillo-Conde B, Lueth P, Oster CJ, Phillips GJ, Narasimhan B, Wannemuehler MJ, Bellaire BH. 2012. Analyzing cellular internalization of nanoparticles and bacteria by multi-spectral imaging flow cytometry. J Vis Exp. (64) e3884, DOI: 10.3791/3884. |
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15. Chavez-Santoscoy A, Huntimer L, Ramer-Tait AE, Narasimhan B, Wannemuehler MJ. 2012. Harvesting alveolar macrophages and evaluating cellular activation induced by polyanhydride nanoparticles. J Vis Exp. (64) e3883, DOI: 10.3791/3883. |
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16. Chavez-Santoscoy A, Roychoudhury R, Pohl NLB, Wannemuehler MJ, Narasimhan B and Ramer- Tait AE. 2012. Tailoring the immune response by targeting C-type lectin receptors on alveolar macrophages using pathogen-like amphiphilic polyanhydride nanoparticles. Biomaterials. 33(18):4762-72. |
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17. Petersen LK, Phanse Y, Ramer-Tait AE, Wannemuehler MJ, Narasimhan B. 2012. Amphiphilic polyanhydride nanoparticles stabilize Bacillus anthracis protective antigen. Mol Pharm. 9(4):874-82. PMCID: PMC3325798. |
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18. Ulery BD, Petersen LK, Phanse Y, Kong CS, Broderick S, Kumar D, Ramer-Tait AE, Carrillo-Conde B, Rajan K, Wannemuehler MJ, Bellaire BH, Metzger DW, Narasimhan B. 2011. Rational design of pathogen-mimicking amphiphilic materials as nanoadjuvants. Sci Rep. 1:198. |
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19. Huang TH, Uthe JJ, Bearson SM, Demirkale C, Nettleton D, Knetter S, Christian C, Ramer-Tait AE, Wannemuehler MJ, Tuggle CK. 2011. Distinct peripheral blood transcriptome responses to Salmonella in pigs differing in Salmonella shedding levels: the intersection of IFNG, TLR and miRNA pathways. PLoS One. 6(12):e28768. |
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20. Carrillo-Conde B, Song E-H, Chavez-Santoscoy A, Phanse Y, Ramer-Tait AE, Pohl NL, Wannemuehler MJ, Bellaire B, Narasimhan B. 2011. Mannose-functionalized "pathogen-like" polyanhydride nanoparticles target C-type lectin receptors on dendritic cells. Mol Pharm. 8(5):1877- 86. |
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21. Petersen LK, Ramer-Tait AE, Broderick SR, Kong CS, Ulery BD, Rajan K, Wannemuehler MJ, Narasimhan B. 2011. Amphiphilic polyanhydride nanoparticle adjuvants activate innate immune responses in a pathogen-mimicking manner. Biomaterials. 32(28):6815-22. |
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22. Ramer-Tait AE, Petersen CA, Jones DE. 2011. IL-2 limits IL-12 enhanced lymphocyte proliferation during Leishmania amazonensis infection. Cell Immunol. 270(1):32-9. |
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23. Liu Z, Ramer-Tait AE, Henderson AL, Demirkale CY, Nettleton D, Wang C, Hostetter JM, Jergens AE, Wannemuehler MJ. 2011. Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger. Dig Dis Sci. 6(10):2838-48. |
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24. Torres MP, Wilson-Welder JH, Lopac SK, Phanse Y, Carrillo-Conde B, Ramer-Tait AE, Bellaire BH, Wannemuehler MJ, Narasimhan B. 2011. Polyanhydride microparticles enhance dendritic cell antigen presentation and activation. Acta Biomater. 7(7):2857-64. |
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25. Ulery BD, Kumar D, Ramer-Tait AE, Metzger DW, Wannemuehler MJ, Narasimhan B. 2011. Design of a protective single-dose intranasal nanoparticle-based vaccine platform for respiratory infectious diseases. PLoS One. 6(3): e17642. |
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26. Boggiato PM, Ramer-Tait AE, Metz K, Kramer EE, Gibson-Corley K, Mullin K, Hostetter JM, Gallup JM, Jones DE, Petersen CA. 2010. Immunologic indicators of clinical progression during canine Leishmania infantum infection. Clin Vaccine Immunol. 17(2):267-73. |
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27. Lei SM, Ramer-Tait AE, Dahlin-Laborde RR, Mullin K, Beetham JK. 2010. Reduced hamster usage and stress in propagating Leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation. J Parasitology. 96(1):103-8. |
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28. Liu Z, Henderson AL, Nettleton D, Wilson-Welder JH, Hostetter JM, Ramer-Tait A, Jergens AE, Wannemuehler MJ. 2009. Mucosal gene expression profiles following the colonization of immunocompetent defined flora C3H mice with Helicobacter bilis: a prelude to typhlocolitis. Microbes Infect. 11:374-83. |
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29. Boggiatto PM, Jie F, Ghosh M, Gibson-Corley KN, Ramer-Tait AE, Jones DE, Petersen CA. 2009. Altered dendritic cell phenotype in response to Leishmania amazonensis amastigote infection is mediated by MAP kinase, ERK. Am J Pathol. 174(5):1818-26. |
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30. Dahlin-Laborde RR, Scolaro EJ, Romine NM, Ramer-Tait AE, Lei SM, Beetham JK. 2008. Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. Ann NY Acad Sci. 1149:347-51. |
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31. Gibson-Corley KN, Hostetter JM, Hostetter SJ, Mullin K, Ramer-Tait AE, Boggiatto PM, Petersen CA. 2008. Disseminated Leishmania infantum infection in two sibling foxhounds due to possible vertical transmission. Can Vet J. 49(10):1005-8. |
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32. Ramer AE, Vanloubbeeck YF, Jones DE. 2006. Antigen-responsive CD4+ T cells from C3H mice chronically infected with Leishmania amazonensis are impaired in their transition to an effector phenotype. Infect Immun. 74(3):1547-1554. |
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33. Vanloubbeeck YF, Ramer AE, Jie F, Jones DE. 2004. CD4+ Th1 cells induced by dendritic cellbased immunotherapy in mice chronically infected with Leishmania amazonensis do not promote healing. Infect Immun. 72(8):4455-63. |
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34. Zoglmann MS, Jones GF, Coffey DM, Stalder KJ, Ramer AE, Deppe MA. 2004. The role of 4-H and FFA involvement and gender on student success in an introduction to animal science course. J Southern Agric Educ Res. 54(1):219-29. |
PUBLICATIONS - Book Chapters |
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1. Overstreet AMC, Ramer-Tait AE, Jergens AE and Wannemuehler MJ (2012). The Role of the Microbiota in Gastrointestinal Health and Disease, Inflammatory Bowel Disease, Imre Szabo (Ed.), ISBN: 978-953-51-0879-5, InTech, Available from: http://www.intechopen.com/books/inflammatorybowel- disease/the-role-of-the-microbiota-in-gastrointestinal-health-and-disease |
GRANTS - Current |
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Impact of the intestinal microbiota on the host epigenome and obesity phenotype NIH COBRE 1P20GM104320, Nebraska Center for the Prevention of Obesity Diseases through Dietary Molecules, Zempleni J (PI) Ramer-Tait AE (Project Leader for Research Project 4) $450,000 in direct costs for 09/01/14 - 08/31/17 |
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Corn-derived resistant starches to improve obesity and metabolic syndrome Nebraska Corn Board Research Grant Ramer-Tait AE (PI) and Walter J (Co-PI) $74,131 in direct costs for 07/01/14 to 06/30/16 |
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Impact of nutritionally enhanced, novel soybean oils on metabolic health and the intestinal microbiota Nebraska Research Initiative Grant Ramer-Tait AE (PI), Benson AK (Co-PI), Clemente T (Co-PI) and Cahoon E (Co-PI) $100,000 in direct costs for 06/01/14 to 05/31/16 |
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Development of DNA-loaded nanoparticles for intestinal gene therapy and DNA vaccination Nebraska Research Initiative Grant Pannier A (PI), Ramer-Tait AE (Co-PI) and Brown D (Co-PI) $100,000 in direct costs for 06/01/14 to 05/31/16 |
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Impact of galacto-oligosaccharide supplementation on susceptibility to enteric bacterial pathogens UNL Faculty Seed Grant Ramer-Tait AE (PI) and Hutkins R (Co-PI) $10,000 in total costs for 01/01/13 - 12/31/14 |
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Impact of Escherichia coli colonization on susceptibility to inflammatory insults Crohn's and Colitis Foundation of America Career Development Award Ramer-Tait AE (PI), Wannemuehler MJ (Co-PI), Jergens AE (Co-PI) and Phillips G (Co-PI) $270,000 in direct costs for 01/01/12 - 12/31/14 |
GRANTS - Completed |
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Nanoparticle-based single dose vaccine against cancer ISU Margaret B. Barry Cancer Research Award Ramer-Tait AE (PI)*, Narasimhan B (Co-PI) and Wannemuehler MJ (Co-PI) $180,000 in direct costs for 01/01/12 - 12/31/14 *PI status transferred to MJ Wannemuehler after Ramer-Tait left ISU, July 2012 |
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Effects of exposure to organic dust on macrophage function: Implication for swine respiratory health Iowa Pork Board Research Grant #11-064 Tuggle C (PI), Ramer-Tait A (Co-PI), Wannemuehler M (Co-PI), Johnson A (Co-PI) and McKean J (Co- PI) $59,218 in direct costs for 12/01/11 - 11/30/12 |
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Understanding gastrointestinal microbial community dynamics in health and disease ISU Bailey Research Career Development Grant Jergens AE (PI), Phillips GJ (Co-PI) and Ramer-Tait AE (Co-PI) $150,000 in direct costs for 07/01/11 - 06/31/14 |
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Elucidating the role of CD4+ T cells during host immune responses to commensal gastrointestinal microbiota ISU College of Veterinary Medicine Seed Grant Ramer-Tait AE (PI), Reecy JM (Co-PI) and Wannemuehler MJ (Co-PI) $12,000 in direct costs for 07/01/11 - 06/31/12 |
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Effects of swine barn dust exposure on macrophage function: implications for swine respiratory health Iowa State University Healthy Livestock Initiative Grant Ramer-Tait AE (PI), Tuggle CK (Co-PI) and Wannemuehler (Co-PI) $10,000 in direct costs for 07/01/10 - 06/30/12 |
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Use of new a new mutagenesis strategy to identify genes important for Salmonella enterica serovar Typhimurium survival in the mammalian host Iowa State University Healthy Livestock Initiative Grant Phillips GJ (PI), Ramer-Tait A (Co-PI), Jergens A (Co-PI) and Wannemuehler MJ (Co-PI). $40,000 in direct costs for 07/01/10 - 06/30/12 |
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Molecular-phylogenetic characterization of microbial community imbalances in colitic IL-10- deficient mice. Iowa State University College of Veterinary Medicine Translational GrantJergens AE (PI), Phillips GJ (Co-PI), Ziemer C (Co-PI) and Ramer-Tait AE (Co-PI) $20,000 in direct costs for 07/01/09 - 06/30/10 |
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Closing the gap between immunology and breeding: Development of high throughput phenotyping technologies to improve disease resistance and food safety Iowa State University Center for Integrated Animal Genomics Research Support Grant Tuggle CK (PI), Wannemuehler MJ (Co-PI) and Ramer-Tait AE (Co-PI) $25,000 in direct costs for 07/01/09 - 06/30/10 |
MENTORING - Graduate Students
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| Student | Degree Sought | Department/Inerdpt Program | Years |
| Hatem Kittana | PhD | Food Science and Technology | 2013-present |
| Carlos Gomes-Neto | PhD | Food Science and Technology | 2013-present |
| Rafael Segura Munoz | MS | Food Science and Technology | 2013-present |
| Elizabeth Cody | MS | Food Science and Technology | 2014-present |
Graduate Student Thesis Advisory Committee (Program of Study Committee) |
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| Student | Degree Sought | Department/Interdpt. Program | Years |
| Mei Liu | PhD | Food Science and Technology | 2011-present |
| Meghan Wymore-Brand | PhD | Immunobiology (Iowa State Univ) | 2011-present |
| Yuan Jin | PhD | Food Science and Technology | 2012-present |
| Maria Maldonado-Gomez | PhD | Food Science and Technology | 2012-present |
| Catherine Mueller | PhD | Food Science and Technology | 2013-present |
| Kwame Andoh-Kumi | PhD | Food Science and Technology | 2013-present |
| Monchaya Rattanaprasert | PhD | Food Science and Technology | 2014-present |
| Junyi Yang | PhD | Food Science and Technology | 2014-present |
| Andrew Demers | PhD | Food Science and Technology | 2014-present |
| Katherine Howard | MS | Nutrition | 2014-present |
| Joseph Roberts | MS | Nutrition | 2014-present |
| Kaye Ivens | PhD | Food Science and Technology | 2014-present |
| Haiyan Sun | PhD | Veterinary Medicine | 2014-present |
| Bharathi Krishnan | PhD | Veterinary Medicine | 2014-present |
MENTORING - Postdoctoral Research Associates and Visiting Scientists |
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| Postdoc/Scientist | Degree(s) Held | Years |
| Hatem Kittana | BVSc | 2012 |
| Laure Bindels | PharmD, PhD | 2013-present |
SCHOLARLY AND UNIVERISTY SERVICE |
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| 2013 - present |
Microbe Maniacs presentations at Culler Elementary (Lincoln, NE) and the USA Science and Engineering Festival (Washington, DC) to teach kids about gut microbes |
| 2013 -present | FDST Search Committee for Gastrointestinal Microbiologist Faculty Position |
| 2013 - present | UNL IANR Committee on Policy for Appointment and Promotion |
| 2013 | FDST Search Committee for Student Recruiter Position |
| 2012-present |
Ad-hoc Reviewer for Microbes and Infection, Molecular Pharmaceutics and Mucosal Immunology |
| 2010 | Search committee for Dean of the College of Veterinary Medicine, Iowa State Univ. |
| 2010-2012 | Reviewer for ISU College of Veterinary Medicine intramural grants |