Natural Product and Food Analysis Facility

Natural Product and Food Analysis Facility
The Natural Product and Food Analysis Facility is an analytical service laboratory, located at the Department of Food Science and Technology, University of Nebraska Lincoln that provides analyses as they pertain to food and other natural systems. Our expertise lies in testing natural products and foods for their exert health promoting agents, i.e., nutraceuticals, while also providing standard food analyses that range from food rancidity testing, shelf life, to proximate analysis. We also provide customized end preparation unique for every product prior to analysis and final data analysis interpretation. These services are provided to our University community, private / public companies, and the general public on a fee-for- service or contract basis. The laboratory is supervised by Dr. Vicki Schlegel who is an analytical food chemist, with research interests in nutraceuticals and functional foods.
Contact: Vicki Schlegel, Ph.D. (vschlegel3@unl.edu), Richard Zbasnik (lab manager), (rzbasnik2@unl.edu).

Service Method Price
General Tests
%Ash Muffle Furnace $25.00
% Fat Folch or Soxlet $75.00
% Fat Milk Mojonnier $65.00
% Protein Bicinchoninic acid assay $40.00
% Protein Combustion $25.00
% Protein Kjeldahl $100.00
Heavy Metals $45.00 - $75.00 each
Minerals (potassium, zinc, etc.) Atomic Absorption or Mass Spectrometry $35.00 - $65.00 each
Moisture Convection $25.00
pH (10% Solution) pH Meter $18.00
pH (direct) pH Meter $18.00
Proximates Fat, Protein, Ash, Moisture, Carbohydrate $110.00
Specific gravity Pycnometer $25.00
Viscosity Viscometer $30.00
Water activity Water Activity Meter $30.00
     
Alcoholic Beverage Analyses
Real Extract $70.00
Alcohol by Volume (ABV) / Alcohol by weight (ABW) $45.00 for each test
$75.00 for both tests
Minerals and heavy metals $35.00 per mineral
$250.00 for whole profile
Water based compounds
(e.g., sulfate, nitrate, nitrite, fluoride, chloramine)
$25.00 per compound
$100.00 for all five compounds
Carbohydrates Included in calories tests
Calories

$110.00, but includes protein, carbohydrates, fat, moisture and ash
$350.00 for those items that require dietary fiber, but will also include protein,  carbohydrates, fat, moisture and ash

Total Sugars $50.00
Bitterness (International Bitterness Units - IBU) $60.00
Diacetyl Values (VDK) $100.00
Aldehydes (common indicator of spoilage) $60.00
Specific Gravity $30.00
Color (Standard Reference Method -SRM)  $50.00

Real degree of fermentation /
apparent attenuation

$65.00 per both tests
Chill Haze $60.00
pH $15.00
Real Attenuation $45.00
Real Extract $70.00
Dietary Fiber (not usually needed for some beers, but FDA requires for restaurants) $250.00
Specialized Sugar and Carbohydrate Tests
Carbohydrates (Total) Phenol Sulfuric Acid $50.00
Degrees Brix   $20.00
Dietary Fiber (Total) Enzymatic/Gravimetric $200.00
FOS (Composition and Total) Ion Exchange HPLC $100.00
Fructans (Total) Enzymatic/Colormetric $125.00
GOS (Total) Gas Chromatography $125.00
Phosphate sugar profiles  (Composition and Total) Ion Exchange HPLC **
Raffinise/Stachyose (Compostion and Total) Ion Exchange HPLC $100.00
Resistant Starch (Total) Enzymatic/Colormetric $200.00
Simple sugar profile (Composition and Total) Ion Exchange HPLC $150.00
Soluble and insoluble Fiber  (Total for Each) Enzymatic/Gravimetric $250.00
Starch (Total) Enzymatic/Colorimetric $75.00
     
Specialized Lipid Tests
Fatty acid profile (Relative amounts of each present unless otherwise specified) Gas Chromatography $165.00
Lipid (Composition) Thin Layer Chromatography $75.00
Lipid (Composition) Gas Chromatography $100.00
Policosanols (Composition and Total) Gas Chromatography $120.00
Short chain fatty acids (Composition and Total) Capillary Electrophoresis or Gas Chromatography $15.00 per acid
Steryl esters / free sterols / cholesterol (Composition and Total) Gas Chromatography $130.00
Tryacylglycerides (Composititional) Colorimetric $60.00
Tryacylglycerides (Totals) High Performance Liquid Chromatography $100.00
Add 15% to the price of these tests if fat has to be extracted.    
     
Specialized Lipid Degradation Tests
Conjugated dienes (Total) Colorimetric $60.00
Fatty Free Acids $60.00
Hexanal $70.00
P-Anisidine value $60.00
Peroxide (Total) Colorimetric $60.00
TBARS (Thiobarbituric Acid) $60.00
Trans Fat (Yes/No) Infrared Spectroscopy $50.00
Add 15% to the price of these tests if fat has to be extracted.    
     
Specialized Protein/Peptide/Enzyme Tests
Amalase (Activity) Colorimetric $65.00
Catalase (Activity) Colorimetric $65.00
Collagen (Total) Colorimetric $65.00
Glucosiadase (Activity) Colorimetric $65.00
Glutathione (Total) Colorimetric $65.00
Superoxidase ( Activity) Colorimetric $65.00
Xanthine Oxidase (Activity) Colorimetric $65.00
     
Phytochemical Tests
Anthocyanins (Total) Absorbance at varying pH $60.00
Chlorophyll A and B (Total of Each) Colorimetric $50.00
Condensed Tannins (Total) Vannilin Method $75.00
Flavanone and dihydroflavonol  (Total) 2,4-dintrophenylhydrazine / Colorometric $60.00
Flavone and flavonol (Total) Aluminum Chloride Method $60.00
Lignan $125.00
Phenols (Total) FARP Method $60.00
Phenols (Total) Folin - Ciocalteu $60.00
Phytic Acid (Total) Wade / Colorometirc $85.00
Resveratrol (Total) Reverse Phase HPLC **
Saponins (Total) Aesin - Vanillin Method $75.00
Selective anthocyanins (Compositional or Total of Select Analytes) Reverse Phase HPLC **
Selective Flavonoids (Compositional or Total of Select Analytes) Reverse Phase HPLC **
Selective phenolic acids (Compositional or Total of Select Analytes) Reverse Phase HPLC **
Specialized extractions Free, Bound, and Conjugated Phenols **
Specialized fractionation Acids, Phenolics, Anthocyanins **
     
Vitamins and Carotenoids
Carotenoids (Total) Colorimetric $60.00
Selective Carotenoids (Compositional or Total of Select Analytes) Reverse Phase - HPLC $75.00-$125.00^
Vitamin A (beta caroteine) (Identification and Total) Reverse Phase - HPLC $90.00
Vitamin A (retinol) (Identification and Total) Reverse Phase - HPLC $90.00
Vitamin C (ascorbic acid) (Indentification and Total) Reverse Phase - HPLC $55.00
Vitamin C (ascorbic acid) (Total) Fluorescnce $100.00
Vitamin E (three isomers) (Total of each Tocopherols) Reverse Phase - HPLC $90.00
     
Free Radical Tests
Antioxidative capacity (hydrophillic agents) Oxygen Radical Absrobance Capacity $60.00
Antioxidative capacity (lipids) Oxygen Radical Absrobance Capacity $65.00
Hydrogen peroxide Colorimetric $65.00
Super Oxide Colorimetric $65.00
     
Other Specialized Tests
Anti-inflammation Capillary Electrophoresis or Mass Spectrometry **
Cytotoxcity / Apoptosis / Necrosis Cell Culture **
Inflammation Cell Culture **
Mammalian / plant amino acids (Compositional or Total of Select Analytes) Cell Culture **
Mammalian / plant nucleotides (Compositional or Total of Select Analytes) Capillary Electrophoresis or Mass Spectrometry **
Mammalian / plant organic acids (Compositional or Total of Select Analytes) Capillary Electrophoresis or Mass Spectrometry **

**  Price negotiated based on analyte(s) to be identified / quantitatated and the sample matrix.
^ Price is dependant on type and number of carotenoids to be analyzed.
 

  • Prices are listed as base price but my change slightly according to food matrix and number of samples, i.e., (10 or more samples, 5-10% discount depending on test).

  • Assays are based either on AOAC, USP, AOCS, or published methods.

  • Prices increase 25-50% based upon turn around times expected.  Typically, turn around times are 6-12 working days from date of sample receival.

  • Tests are performed in triplicate analyses per sample (in most cases).  Fiber tests or other tehcnoical tests  are performed in duplicate.

  • All HPLC, gas chromatography, and technical instrumental tests require at least 3 samples for analyses.

  • Prices are discounted for UNL Faculty and Staff -- Request Information

Contact: Dr. Vicki Schlegel - vschlegel3@unl.edu | Richard Zbasnik (lab manager), (rzbasnik2@unl.edu).